Amphiregulin confers protection to murine hematopoietic progenitor cells from apoptotic deat
Sushmita Roy, Shyam Krishnan, Vikas Kumar, Ashwani Kesarwani, Asok Mukhopadhyay*Abstract: Amphiregulin (Areg) is a unique ligand of epidermal growth factor family. The functions of Areg in hematopoiesis remain to be elucidated. Using mouse bone marrow (BM) regeneration model and in vitro culture systems, we were able to show that Areg is a potent anti-apoptotic factor that rescues hematopoietic stem and progenitor cells (HSPCs) from apoptotic stimuli. Like other growth factor genes, Areg was found to be up-regulated in the stromal compartment (CD45-) of BM. The role of Areg was determined by loss-of-function study using shRNA-mediated Areg knock-down M210B4 (M210B4Areg(k/d)) stromal cell line. Coculture experiments of BM Lin-Sca-1+c-Kit+ (LSK) cells with wild-type or M210B4Areg(k/d) stroma revealed that a significant fraction of LSK cells in knock-down stroma progressed towards apoptosis due to auto-activation of death receptor CD95R and subsequent activation of pro-apoptotic (Bad, Bax) and suppression of anti-apoptotic (Bcl-xL) genes, and activation of caspases-3 & 7. The role of Areg was confirmed by reconstitution assay where M210B4-conditioned medium significantly recovered LSK cells from apoptotic death.View PDF
Facing Mesenchymal Stem Cells in Psoriasis and Chronic Atopic Dermatitis: More Similar Than Different?
Campanati Anna1, Caffarini Miriam2, Sorgentoni Giulia2, Ganzetti Giulia1, Offidani Annamaria1, Di Primio Roberto2, Orciani Monia*2Abstract: Both atopic dermatitis (AD) and psoriasis are chronic, immunomediated and inflammatory skin diseases, which have been traditionally considered the two opposite poles of the Th1/Th17 versus Th2 paradigm, showing specular differences in cytokinic milieu. While for psoriasis a greater expression of Th1-Th17 chemokinesis usually detected, for AD the question is more complex: it is initially characterized by a Th2 profile (acute phase), with a shift to Th1/Th17, or a mixed Th1-Th2 pattern, during the chronic stage.We have already demonstrated that MSCs derived from tissue affected by psoriasis showed a different immunobiology from those from healthy subjects, and this finding leads to hypothesize that MSCs could be involved in the early phase of psoriasis pathogenesis.View PDF
Nursing Management in Autologous Hematopoietic Stem Cell Transplantation for Multiple Sclerosis and Type 1 Diabetes Mellitus
Loren Nilsen, Lais Carvalho Castanho, Patrícia Scotini Freitas, Maria Carolina de Oliveira Rodrigues, Cristina Maria Galvão, Renata Cristina de Campos Pereira SilveiraSilveira*Abstract:View PDF HTML View
Introduction : To identify clinical symptoms and nursing interventions during the conditioning period for autologous hematopoietic stem cell transplantation, while using antithymocyte globulin, in patients with multiple sclerosis who were receiving steroids and in patients with type 1 diabetes mellitus who were not receiving steroids. Patients with serious and progressive autoimmune diseases, such as multiple sclerosis and type 1 diabetes mellitus, that often develop resistance to conventional therapies with immunomodulatory and anti-inflammatory agents, which are rendered insufficient to impede irreversible damage to the affected systems. New therapeutic approaches, such as autologous hematopoietic stem cell transplantation, have been developed that offer promising global survival and progression-free levels.
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